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These pre-clinical studies attributed the recovery in symptom profile to modulation of glucose transport, over-expression of mitochondrial genes, bypassing defective pathways of ETC (electron transport chain), and improvement in mitochondrial biosynthesis, modulation of microbiota, regulation of brain neuronal networks, and promoting synthesis of white matter in the brain [168]

All cell lines were kept in a humidified 37 C/5% CO 2 incubator, except Expi293 cells that were kept in a humidified 37 C/8% CO 2 incubator on an orbital shaker (125 rpm)

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